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Combining low-cost foreground and recombinant selection (using KASP markers) with high-resolution background selection (using the C6AIR) provides a powerful strategy for rapid and precise MABC transfer of introgressions into elite genetic backgrounds.
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Markers for background selection were selected using the strategy outlined in Figure 5, and by using gene and recombination distribution information provided by Erayman et al. (2004).
Method for the background and foreground 3D assignment and selection using a binary image obtained by using the image reference method.
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Foreground selection using QTL specific markers, recombinant selection using flanking markers, and background selections were performed in every generation.
Univariate comparisons by rural vs urban background were made using the χ test for categorical variables and unpaired T-test for each selection factor.
Reddy et al. [22] proposed a block selection approach using the discrete cosine transform (DCT) among some neighboring blocks to estimate the unconstructed parts of the background.
A total of 248 SSR markers were used for background selection of the three ABL along with the BB-resistant donor line IRBB57 and a genetic map covering a 1,446.6 cM region of the O. sativa genome was constructed (Figure 3).
Background selection was performed using 65 well-distributed SSR markers to preserve most of the KDML105 genetic background for the adaptability and market quality of the Thai Jasmine rice (Table 2).
Background selection was performed using ~30 simple sequence repeats (SSR) markers randomly distributed along the maize genome, which allowed for the selection of NILs with ~98% of the L53 genome.
The statistical significance for this selection procedure compared with the background rate was evaluated using the Poisson probabilities as previously described in the reference.
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