Sentence examples for background fluorescence using the from inspiring English sources
All our analyses involved the manual removal of background fluorescence using the MxPro - MX3000P v4.10 (Stratagene) after each run.
Intensity thresholds were set to include all pixels equal to or greater than the intensity of the mean background fluorescence using the separation feature and restrictions set for puncta size (Fig. S2).
Fluorescence intensity of the BiFC signal was measured by selecting cells positive for both Nef and Flag-tagged MHC-I, then subtracting the background fluorescence using ImageJ55,56.
FTIR and Raman spectroscopies proved to be useful techniques for the identification of the mineral composition of these archaeological materials, although the latter one showed some limitations due to the presence of non-desired background fluorescence using a 785 nm laser.
In each experiment, coverslips were viewed with a 63× objective Zeiss LSM510 fluorescence microscope and confocal images were acquired using identical photomultiplier settings and corrected for background fluorescence using unlabelled specimens.
Images were corrected for background fluorescence using unlabelled specimens.
Fluorescence changes (∆F/F) for each odor were calculated in relation to background fluorescence using frames 0 6 (i.e., 2 0.5 s before odor application).
DHE intensity was analyzed by confocal microscope and quantified by counting the number of pixels exceeding a specified threshold, which was set in order to eliminate interference from any background fluorescence using ImageJ software.
To determine the background level of fluorescence, using the Cluster 3.0 program, the expression data was log2 transformed, mean centered, and normalized across the time course for each gene.
Crossing points, defined as the point at which the fluorescence rises above the background fluorescence was determined using the "Fit Point Method" in the LightCycler software 3.5.3 (Roche Diagnostics).
IF images were obtained at a constant exposure time to limit overexposure, and α-tubulin IF signals radiating from γ-tubulin positive centrosomes were measured in two separate concentric circles centred at the centrosome with radii of 1 and 2 μm, with background fluorescence subtracted using circles of corresponding sizes, using IF Jpeg images and ImageJ software [ 36].
