This was shown by both, flow cytometric (Fig. 1b) and ELISPOT analysis (Supplementary Fig. S1).
These sequences were aligned and used for phylogenetic analysis (Supplementary Figure S1).
We integrated TKI binding data with our differential phosphorylation analysis (Supplementary Table S2).
For IGFBP3, a large region across the gene was considered noteworthy using a sliding window analysis (Supplementary Figure S3).
AIP1 was estimated to consist of 0.027% of total proteins in this fraction by immunoblot analysis (Supplementary Fig. S1).
First, for each primary and secondary analysis, supplementary statistical models were fit, comprising alternative combinations of covariates.
These findings were confirmed on multivariate analysis (Supplementary Table S2).
Similar results were obtained from bootstrapping analysis (Supplementary figure 1).
This was not significant in multivariate analysis (Supplementary Table 3).
Phylogenetic analysis (Supplementary Figure S1B) and bootscanning analysis (Supplementary Figure S1C) indicated that pCRF07_BC was indeed a CRF07_BC subtype as described previously.
Results obtained by ELISA were confirmed by western blot analysis (Supplementary Figures 1 3).
