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In both datasets, survival curves for individual miR-564 targets were also analyzed (Supplementary Fig. 5A,B).

Although the Sanganji Jomon data were merged for sequence data of A1 and B, the PCA plot location of the Sanganji Jomon was similar to Figure 1b when A1 and B were independently analyzed (Supplementary Figure S13).

In the HDAC4ΔC mice no neuronal cell loss is apparent in any of the brain regions analyzed (supplementary figure S1).

Similar results were observed when the percentage of Ki67 positive cells was analyzed (Supplementary Figure 4).

A panel of 19 antibodies related to TCR and CD30 pathways was analyzed (Supplementary Table 1).

In total, 1010 deleterious natural splice site variants were analyzed (Supplementary Table 4).

NGS data were processed and analyzed uniformly, Supplementary Methods, Supplementary Figure 3, Supplementary Table 1, and associated genomics results.

Calculations of ANI were conducted between all analyzed strains (supplementary table S1, Supplementary Material online).

This involved an average increase of 11.13% in the number of analyzed codons (supplementary table S3, Supplementary Material online).

The same pattern of results was observed when we collected the triose phosphate isomerase genes and phylogenetically analyzed them (supplementary file S2, Supplementary Material online).

In both wild and genebank H. spontaneum, only very few haplotypes per locus (mostly one to two) reached higher frequencies (>20% of the analyzed samples; supplementary table S3, Supplementary Material online).