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In addition, 7,438 genes with normalized counts of <3 for >75% subjects were excluded leaving 15,681 genes for analysis (Supplemental Figure S1C).
All cells used in this study had normal appearing cellular and subcellular morphology as assessed by cytoskeleton analysis (Supplemental Fig. 2B).
Four genes (gch, pvalb, hoxb6, dlx2) were validated through whole mount in situ analysis (Supplemental Fig. S1) and 28 other genes were further validated through quantitative RT-PCR analysis (Supplemental Tables S1 and S2).
Our results were not appreciably affected when 12-year-old subjects (n = 3) were excluded from analysis (supplemental Figures S3 and S4).
PCR results for 8 genes were not conclusive even after 2 repeats and the genes were removed from further analysis (supplemental Table S3).
Western blot analysis (supplemental Figure S2) showed that the allosteric antibody WC75 and the ligand competitive antibody WC613 both immunoprecipitated Notch1 but not Notch4.
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For additional details showing the results of our MDL substitution analysis, see Supplemental Material and Supplemental Figure 1 (http://www.ehponline.org/members/2009/11861/suppl.pdf).org/members/2009/11861/suppl.pdf
Data collection involved content analysis and supplemental surveys.
Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures).
The accumulated-wealth section of the analysis provides supplemental information on equity gains, stock holdings and retirement plan accounts.
The accumulated-wealth section of the analysis includes supplemental information on equity gains and retirement plans.
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