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A hybrid amplification design consisting of distributed Raman amplification and EDFA (Raman/EDFA), which has led to significant enhancement in system performance, is also described.
This chapter presents a new strain amplification design, named a "nested rhombus" multilayer mechanism, that increases strain exponentially through its hierarchical cellular structure.
This study can provide a theoretic foundation for the amplification design of the moving-bed reactor in the poly-generation system.
Thus, the proposed PCR-mediated amplification design could be a facile method for identifying the protease gene profiles as well as for detecting novel protease genes of the B. thuringiensis strains.
To develop a new MB sensing system and simply the signal amplification design, we herein attempted to propose a multifunctional integrated MB (MI-MB) for the polymerization amplification detection of target DNA via introducing a G-rich fragment into the loop of MB without using any exogenous auxiliary oligonucleotide probe.
Modifications to PCR amplification design have allowed robust 50-plex genotyping from as little as 5 ng of DNA, with 100% call rate and >99.9% accuracy.
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The NR2A, NR2B, NR2C, NR2D, NR3A, and GAPDH mRNA transcripts were quantified using the oligonucleotide primers for real-time PCR amplification designed based on sequences published in the Harvard Primer Bank and the NCBI primer-designing tool where the sequences were blasted.
MR made the whole genome amplification, designed some markers, made and analyzed the genotyping.
Exome sequencing results for prioritized variants were validated using specific primers for polymerase chain reaction amplification designed using Primer3Plus (http://primer3plus.com/cgi-bin/dev/primer3plus.cgi) and Sanger sequencing (GATC Biotech, Cologne, Germany).
Briefly, 500 ng of DNA isolated from the prostate were subjected to PCR amplification designed to detect polyomaviruses and HPV [ 4, 8, 11].
Primers for all amplifications, designed with PerlPrimer v1.1.10 (freeware by Owen Marshall), were located on exon-exon borders to prevent amplification of potentially contaminating genomic DNA.
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gain design
amplifier design
scalability design
chain design
extension design
increase design
pcr design
escalation design
enhancement design
scaling design
scale up design
expandable design
widening design
expanding design
amplified design
amplification protocol
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amplification role
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