A bi-amplified design – which includes a high-performance signal processor, class-D amplifier and digital crossover – delivers crystal clear sound with minimal noise and distortion.
The Casp8p41 construct was PCR amplified by designing a reverse primer with a stop codon at amino acid 356 (5'-TTAAAACACTTTGGGTTTTC-3') coupled with a sense primer beginning at the initial ATG (5'-ATGGACTTCAGCAGAAAT-3') and ligating into the pGEM-T Easy vector (Promega, Madison, WI) for subsequent cloning.
cDNA was amplified using GE Illustra Hot Start RTG beads and amplified using primers designed for the tested genes.
The Logitech Speaker System Z520 brings your music, movies and games to life thanks to an amplified two-way design and 26 watts (RMS) of power.
The deformations of the blocks in ultrasonic range are internally amplified via the design of the motor by about 80 times and collectively yield an elliptical trajectory for the driving head of the motor.
5S rRNA intergenic regions of Setaria digitata and Setaria labiatopapillosa were PCR amplified with primers designed from the 5S rRNA gene of Brugia malayi.
Two regions flanked by genes trnY and rns and trnW and cox2 were identified by comparing the whole mitochondrial genomes of Phytophthora infestans, Phytophthora ramorum, and Phytophthora sojae and amplified using primers designed from the flanking conserved genes.
Primers that amplified p19Arf were designed: sense, 5'-GGGCCGCACCGGAAT-3' and antisense, 5'-AAGAGCTGCTACGTGAACGT-3'.
The XCC3358, XCC1990 and XCC3209 promoter regions (see Table S6) were PCR amplified with appropriately designed primers.
To generate the UAS>Rbf1N-RFP vector, the mCherry RFP [30] coding sequence was PCR amplified with primers designed with 5' SpeI and 3' XbaI sites: 5'-ATAGTAGTATGGTGAGCAAGGGCGAG-3' and 5'-GCTCTAGATTACTTGTACAGCTCGTCCAT-3'.
All the coding exons and 30bp of the flanking intron-exon boundaries of TARDBP (NM007375.3) were PCR amplified using primers designed using Primer3 software (available upon request) and Roche FastStart PCR MasterMix polymerase (Roche Diagnostics Corp., IN).
