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By comparing pathway recovery for individual reference genomes to pathway recovery for combinations of reference genomes, it becomes formally possible to use Pathway Tools to identify distributed metabolic pathways that emerge between multiple interacting partners.

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In addition, LANA interacts with the DNA methyl binding protein MeCP2, the mSin3 transcriptional repression complex and the histone methyltransferase SUV39H1, thus enabling numerous roles in epigenetic gene regulation (see Shamay et al (2006) for individual references).

The inter-species neutrality tests are able to detect selective events that took place up to a few million years ago (i.e. adaptive events that participated in the emergence of modern human) and include the traditional Ka/Ks, dN/dS and MacDonald-Kreitman (MK) tests (for individual references and detailed reviews of all these tests, see (Bamshad and Wooding 2003; Nielsen 2005; Sabeti et al. 2006).

A graphic overview of BLASTX hits along the query is displayed on top for each individual reference, as exemplarily displayed here for the top part of the output in the Physcomitrella patens reference tab (Fig. 4).

Relative estrogenic potencies were calculated for the individual reference chemicals by dividing the median effect concentration for E2 by the concentration of each chemical (determined using Equation 2) required to cause the same level of effect.

A set of qRT-PCR primers with high-efficiency for nine individual reference genes was designed using the Primer3 software (version 0.4.0) [ 24].

That is, levels of growth hormone (GH) and insulin like growth factor-1 (IGF-1), melatonin (nocturnal), TSH, thyroid hormones (T3), calcitonin, DHEA (sulphated form in and its urinary 17-keto-metabolites), aldosterone, estrogens, testosterone in men and women have been demonstrated to progressively decrease with age in adults (for extensive review and individual references refer Hertogue 2005).

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