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Fractions were concentrated using Vivaspin centrifugal concentrator (Sartorius).
Viral particles were concentrated using Lenti-X concentrator (Clontech).
The purified proteins were concentrated using Apollo centrifugal concentrators (Orbital Biosciences, Topsfield, MA).
The supernatant was collected and lentiviral particles were concentrated using Lenti-X Concentrator (Clontech, CA).
Crude oligosaccharides from charcoal fractionations were concentrated using a SpeedVac concentrator (SC210P1-115, Thermo Scientific) before SEC fractionation.
The supernatant was collected and lentiviral particles were concentrated using a Lenti-X Concentrator (Clontech).
The resulting viral supernatants were concentrated using Amicon 100K cut-off concentrators (Millipore).
To obtained high concentrations for characterization, the proteins were concentrated using a spin column with MWCO 3,000 Da (Millipore).
Supernatants were concentrated using Fast-Trap Lentivirus Purification and Concentration Kit (FTLV00003, Millipore).
Samples were concentrated using Amicon filters to a final subunit concentration of 6 μM.
The supernatants were concentrated using Centrifugal Filter Units (Millipore).
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