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Labeling for neuroglian was primarily in the 25% sucrose fraction, with weaker labeling for the 30−35% interface and the 40+60% fraction (not shown).

In contrast to the staining in the crypts, the apical region of the villi showed a weaker labeling for PLEKHA7, whereas the labeling for ZO-1 was of similar intensity throughout the villus (arrowheads in Fig. 3F-F").

In contrast, direct labeling of MBP-fGly with AO-AF488 produced weaker labeling under similar conditions.

PPR labeling was particularly intense in the neuronal nucleus (Figs.  3j l, 4j l), although weaker labeling was distinguished at the cell surface and in the cytoplasm.

In Trypanosoma brucei trypomastigotes constitutively expressing TbSAS6L-YFP from the endogenous locus, TbSAS6L-YFP localizes near the base of the flagellum (Fig. 4A to C). Label appears as one or two prominent dots, depending on the cell cycle stage, and there is also weaker labeling in the proximal region of the axoneme, in the region of the flagellar pocket collar.

Weaker labeling is observed in the pyramidal cell layer of CA3 and a small segment of CA1.

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Similar(52)

Positive labelling for aggrecan core protein, 6B4, was observed intensely in the outer regions of the pellet with weaker labelling demonstrated in central regions (Fig. 4K).

After 8 h p.i., nuclei showed weaker labelling and a modified appearance: newly synthesized viral particles could be observed as single dots in the cytoplasm.

As shown in Fig. 5, numerous nerve cells in spinal cord were labelled 4 h after the injection, with weaker labelling at 2 h.

Additionally, this intense labelling of esterified pectin in tricellular junctions remained up to maturation in 'Scifresh' fruit, whereas mature 'Royal Gala' fruit displayed weaker labelling.

Antibody PG5 strongly stained cortical neurons and astrocytes, with weaker labelling of neuropil threads in dentate gyrus and molecular layer of the hippocampus.

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