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Transgene integration was verified by mPCR in all primary transformants (T0), while immunoblot analysis confirmed that the protein HrpZPsph was produced and the signal peptide was properly processed.
Immunoblot analysis of all sixty T1 transgenic lines indicated that the protein was indeed produced and the signal peptide was properly processed as shown in Figure 3 for a representative T1 line.
This confirmed that the transit peptide fused to ORFH79 was properly processed and that ORFH79 was imported into mitochondria in these transgenic lines.
To remove breathing or pulsation induced stripe patterns on the en face image, a FFT-based low-pass filtering was properly processed to the en face image.
In both HEK-293T and TOV-112D cells, wtMET was properly processed to an α- and β-chain, indicating that these cells are not defective in furin-mediated processing.
Whereas the extracellular domain of wtMET was properly processed and secreted into the culture medium (as illustrated by the presence of the 309-929 biotinylatextracellularlar β-chain and the MET25-308 α-chain, Fig. 4e, lane 4), no secreted MET products were found in medium of cells, transfected with the METΔ7 8 ectodomain (Fig. 4e, lane 3).
Similar(54)
Abdel Hakim Ben Bader thought that he had applied for his green card, but learned when he went to register that the application had not been properly processed.
IT specialists talked about "patches", to integrate new products into these myriad systems, and about "workarounds", improvised solutions when an activity or product cannot be properly processed.
Milk is an excellent growth medium for numerous bacteria, and the bacteria can increase rapidly in numbers unless the milk is properly processed.
Narayanasamy said the Australian government's offshore detention policy had failed and all of the people held on the islands must now be brought to Australia to be properly processed.
Agency officials say they have begun an aggressive oversight effort to determine if all disability claims are being properly processed and contracted for a study that will examine state-by-state differences in average disability compensation payments.
Related(20)
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