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Interestingly, when expressed in Cos-1 cells, although mito81 210 was correctly processed, the level of mature frataxin was decreased, and the precursor form accumulated within the cytosol (Fig S5F), suggesting that amino acids 42 80 of human frataxin are partially involved in the mitochondrial targeting.
Moreover, the signal peptide of CvChi45 was correctly processed to produce a mature extracellular enzyme with chitinolytic and chitin-binding activities.
It was one of the first examples illustrating that insect cells were appropriate hosts for the expression of plant genes: ABP1 was correctly processed and glycosylated in the endo-membrane system of insect cells.
The largest and most complex protein successfully produced to date is the human clotting protein factor VIII, a large heterodimer that was correctly processed into a bioactive protein in pigs [ 67].
A short hairpin encoding miR-122 cloned into the expression vector was correctly processed to yield mature miRNA upon induction with ligand and the amount of miRNA produced was commensurate with the concentration of ligand.
Sequencing revealed that overexpressed miR-146a was correctly processed, with the most prevalent isomiR being the same in all cell and EV libraries and represented by approximately half of the reads.
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Depending on the extent of ER processing, some fraction of the protein can be correctly processed, trafficked to the lysosome and retain functionality, even in individuals carrying disease-associated mutations.
In this study we use the MHC-pathway model [ 14], which allows us to screen all possible peptide fragments of 14 amino acids within a particular protein for their ability to be correctly processed by the proteasome and transporter associated with antigen processing (TAP), and presented by the MHC class I molecules.
You were eligible for the promised payout, it decided, but a "routine check" to ensure "exceptional service" showed the transfer of £220 had not been correctly processed and might not have been received.
The chimeric heavy and light chains containing red fluorescent protein as a bridge were correctly processed and secreted into Escherichia coli periplasm for assembly and disulphide bond formation, further analysis revealed the molecules to be exclusively monomers.
Both ADAMTS-Fc fusions bound to α2-macroglobulin, further indicating that they were correctly processed with the catalytic activity in this system; however, they failed to digest the peptides derived from the aggrecan sequences known to be clipped by the native enzyme, possibly due to the lack of required multiple interactions existing between the native protease and physiological substrate.
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