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SAMtools was used to characterize sSNVs in these samples; Sanger sequencing was utilized to validate functionally important sSNVs.
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Sanger sequencing was used to sequence five loci that have been previously utilized to infer squamate phylogenies [ 30, 43, 45].
PCR and Sanger sequencing was performed at ACGT.
Sequencing was performed using the Illumina system; Sanger sequencing was used to validate all identified mutations.
Sanger sequencing was used to verify the presence of the W112A mutation.
The rate of occurrence of such SNPs that were further validated by Sanger sequencing was low.
Direct Sanger sequencing was performed for PCR products from both ends.
Sanger sequencing was performed to amplify target regions in PDX #13, #19, #54, and #56.
Sanger sequencing was used to confirm pyrosequencing results.
Obviously the Sanger sequencing was developed based on Wu's primer-extension method.
Sanger sequencing was used to assess small variants of interest in our dataset [28].
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