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In order to screen for molecular differences between embryonic gonads from homozygotes and wild-type embryos more generally, we used spotted DNA microarrays.
For instance, Delsite et al. [7] used spotted radioactive microarrays to compare the transcriptome of a human breast cancer cell line and its ρ° derivative and found deregulation of a number of different genes involved in pathways as diverse as cell signalling, metabolism, cell growth and differentiation.
We used spotted membrane DNA array screening to identify gene expression changes associated with FBN1 mutations.
For this, we used spotted DNA microarrays containing probes for the complete gene set of the S288C reference strain.
Recently, Mercier et al. used spotted microarrays to identify approximately 1400 genes with a significant change in transcription level after exposure to 200 Gy of ionizing radiation [ 34].
We used spotted membrane DNA macroarrays to identify genes whose altered expression levels may contribute to the phenotype of the disease.
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Samples were analyzed before and up to 1 hour after the shock using spotted microarrays.
It was fabricated using spotted oligo technology, which limits the number of probes that can be included on one array.
One study using spotted membrane macroarrays found that 104 of the 1,185 genes tested were differentially expressed in AAA [ 6].
For example: try using "spot the difference" pictures.
If it is difficult to see your baton, try using spotting tape, or electrical tape, on your baton.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com