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The culture supernatants were then collected, centrifuged at 1200 rpm for 7 min at 4°C to remove cellular debris and stored at −80°C until future use.
The samples were stored at −80°C until future use.
The supernatant was collected and stored at -80°C until future use.
All the samples were collected in a tube containing RNA later solution (Qiagen) to preserve the RNA from degradation and kept at -80°C until future use.
Tomato or tobacco pistils were harvested from mature flowers, the ovaries cut away and the remaining stigma/styles stored at -80°C until future use.
The RT-PCR temperature program was adjusted to run for 30 min at 16°C, 30 min at 42°C, 5 min at 85°C, and then 4°C until future use.
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Samples were set at 100 µl with demineralized water and stored at −20°C until future used.
At the very least, the automaker retains ownership until the future use is determined, Mr. Holmes said.
Several sites were concerned about jurisdiction and lack of specificity in being asked to review infrastructure; one site indicated that "we shouldn't be collecting data until we know what the future use might be...this is just a little too wide open, it's a fishing expedition".
NGS libraries will be stored at −20°C until the pregnancy outcome is known for future use if repeat NGS testing is required.
The cells were transferred to 35 cm dishes and cultured for 3 4 days until confluent, and then the cells were frozen for future use.
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