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Inhibition of Tor2 signaling results in the induction of genes involved in sexual differentiation, and the cells undergo mating and meiosis, even under good nutritional conditions.

Because h strains are able to undergo mating type switching, h cells can be induced to self-mate and create homozygous diploids that then proceed through meiosis and sporulation.

Because sporulation requires a diploid DNA content, the species with a haploid lifestyle, such as Z. rouxii, must first undergo mating between heterothallic a and α cells in response to osmostress.

These populations are a heterogeneous mix of architectures, weights, and input variables which undergo mating, crossover, and recombination to ultimately identify an optimum NN solution, simultaneously finding influential SNPs and fitting networks weights.

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No additional MAT gene could be detected in the entire K. capsulata genome, suggesting that haploid strain CBS 1993T is of mating-type alpha and cannot undergo mating-type switching.

As the majority of wild yeast are homothallic this may be particularly important because an unmated fast-germinating individual is also likely to undergo mating-type switching and subsequently mate with its own daughter cell, eliminating it from the pool of potential mates.

Whether T. congolense also undergoes mating is unclear because the current evidence on this question is limited.

They may do so by undergoing mating type switching such as in the brewer's and fission yeasts and some filamentous ascomycetes.

The two MAT genes are flanked by homologs of SLA2 and DIC1, as previously observed in other yeasts (Torulaspora delbrueckii and Lachancea kluyveri), a gene arrangement considered as ancestral and prone to evolutionary erosion in yeast species undergoing mating-type switching (Gordon et al. 2011).

Moreover, methylene-blue vital-staining showed that about 10% of the ccw12Δ cells undergo mating-induced cell death (versus 1% of wt cells).

Indeed, downregulation of either gene alone is sufficient to prevent the mating-induced changes in intestinal progenitors: a finding that we confirmed by observing that viable Met 27 mutants also fail to undergo mating-induced remodelling (data not shown).

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