We conducted mating assays after five days of cage life and again after three weeks.
We randomized both the ages of flies, within the 3-to 6-d age window, and the days on which we conducted mating assays for particular pairwise tests.
To this end, we conducted mating experiments using triplets consisting of a single small and standard-sized male and a small or standard-sized female and characterized the mating behaviour (mate choice, mating latency, duration and frequency) and the intensity and direction of male male competition and female choice within the triplets.
Data were analyzed by GLM in JMP 9.0 (SAS Institute Inc .. We conducted mating trials to determine how differences in the sizes of 2 competing spores of the same mating type affected their likelihood of mating with a single focal cell of the opposite mating type, on both rich and poor medium.
3D printing technology is creating opportunities to conduct mate choice experiments in the field by facilitating the production of biologically accurate decoys.
By linking urine release with aggressive behaviours females generate conditions that allow them to conduct mate choice.
To test the female fertility after germ cell-specific Atg7 knockout, a breeding assay was conducted by mating Atg7 F/F or Atg7 F/F ;Tnap-cre female mice with Atg7 F/F males for 6 months.
To examine premating reproductive barriers between the two species, we conducted mate choice experiments involving both con- and heterospecific partners.
To investigate the consequence of an induced immune response on mating success in H. virescens females and males, we conducted mate choice experiments.
Often, the correlation between cue diversity, evolution, and mating cannot be made directly due to the difficulty in characterizing the mating cue, identifying the gene(s) giving rise to the (often extremely complex) chemical signal, and conducting mate recognition studies in the same populations.
