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Exact(4)
The software in different searches may position the modification to different sites when there is insufficient information for unambiguous site determination and neither assignment contradicts the data.
The selection of appropriate energy deposition methods, however, is necessary to ensure generation of sequence ion series required for unambiguous site assignments.
The method provides information on the intact mass of the protein variant as well as on fragment ions that allow unambiguous site assignment of methionine oxidation necessary for site-specific quantification of methionine oxidation via UV.
Conditions that provide information on the intact mass of the protein as well as on fragment ions that allow unambiguous site assignment of methionine oxidation in filgrastim variants as low as 0.12%% of total peak area in a chromatographic time scale were identified.
Similar(56)
Copy number can be significantly lowered by meganuclease-mediated transgenesis [20], but unambiguous site-specific recombination requires single copy insertions which can only be achieved by pseudotyped retrovirus [21] or transposon-mediated transgenesis [22].
The program uses the alignment in question and returns files with informative and unambiguous sites as well as a log file containing the PHI p-values.
Well-defined signals were observed only for (AAG 5, (ACT 5 and (AAT 5, which appeared restricted to a few unambiguous sites on specific arms of polytene chromosomes.
Finally, we restricted the data to windows with a minimum of 10,000 unambiguous sites, of which there were 1005 in the chicken reference and 1655 in the human reference.
We found that unambiguous sites are always more compatible with the relaxation (or loss) of the functional constraint scenario, and that all three Drosophila OBP proteins would exhibit functionally diverged relaxed positions.
For each dataset, we restricted the data to windows with a minimum of 10,000 unambiguous sites, of which there were 1,038 windows of size 1 Mb, 2,040 of size 500 kb, 3,986 of size 250 kb and 9,205 of size 100 kb.
A total of 92% of the unambiguous sites of the rhesus macaque draft genome was covered with high-quality reads, and the genotype was called with high confidence (genotyping quality score ≥45 and coverage ≥10), which indicated that the rhesus macaque genome would work as a reasonable reference sequence for analysing the Vero cell genome.
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