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Exact(31)
Cytoplasmic extracts were fractionated through sucrose gradients, with the lightest components appearing at the top (fractions 1 and 2), small (40S) and large (60S) ribosomal subunits, and monosomes (80S) in fractions 3 6, and progressively larger polysomes in fractions 7 12.
After centrifugation, 3 ml of top fractions were mixed with 0.5 ml of biuret reagent [9] and used for the measurement of optical density (λ = 550 nm) on "Shimadzu UV-1800" spectrophotometer.
Additional file 3: A tar archive containing the details of the enrichment results, including the individual query (uniquely retrieved actives and decoys at the 1% and 0.25% top fractions), averaged enrichment factors for all of the sets, number of unique ligands and decoys and the number of generated conformers.
Rn, Re and Rt represent the coverage rates of proteins in the nucleoid, envelope, and top fractions, respectively.
Most of the sample material was focused in the middle fractions with exception of the plastidic compartment enriched within the top fractions (data not shown).
In contrast, the distribution of the protein detected only in the envelope and/or top fractions showed that only 1 normal distribution appeared whose peak was around 7.5%.
Similar(29)
Finally, we calculate ACT-50 %, which corresponds to the top fraction of the ranked library that contains half of the active compounds.
Effect of pH on recovery, PF, KE, and VR of alkaline protease in the PEG-rich top phase compared to those values of the top fraction of previous step was depicted in Figure 2.
To increase the protease recovery and purity, additional sodium citrate 10, 15 and 20% (w/w) was added to the first cycle top fraction from 70% (w/w) CE with 15%PEG2000-15PEG2000-15%itrATPSATPS.
These include enrichment factors calculated for the top 1 and 10%% of the ranked library (EF1 % and EF10%%), the Boltzmann-Enhanced Discrimination of Receiver Operating Characteristics (BEDROC20) score, the area under the enrichment curve (AUC), and the top fraction of the ranked library that contains 50%% of the active compounds (ACT-50ACT-50%
Virtually all the "growth" went to the top fraction of 1percentt.
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