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Once the tumors reached a suitable size of 400 to 500 mm3, they were fragmented into roughly equal size of 2×2-mm tissue chunks and implanted into the left and right flanks of the experimental BALB/c nude mice.
Frozen tissue chunks were pulverized under liquid nitrogen, and homogenized in 10 vol of buffer (0.25 M sucrose, 1 mM EDTA [pH 7.4]).
Briefly, frozen tissue chunks were pulverized in 10 vol of lysis buffer (in mM: NaCl 145, MgCl2 0.1, HEPES 15, EGTA 10, pH 7, Triton X-100 0.5, with protease inhibitor cocktail), and solubilized for 30 min on ice.
For RNA isolation, normal and tumor tissue chunks were collected in RNAlater (Qiagen, Hilden, Germany).
Superoxide production has previously been shown to be increased in intact LV myocytes [43], LV homogenates [20] or tissue chunks from nNOS−/− mice [36].
At the end of the procedure, the large prostatic tissue chunks are reduced to small pieces using a soft tissue morcellator to allow evacuation from the bladder [ 1].
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Overall, 769 individual CTCs from blood, 75 DTCs from bone marrow, and 60 single TCs from fresh primary tumor tissue (chunk and core needle biopsy), lymph node metastasis, and a bone metastasis to the spine were collected.
Herbivores, which must shred and pulp the hard-to-digest cellulose cell walls of vegetation before swallowing, tend to have low crowns with many small facets giving them a high OPC, whereas carnivores, which slice easily digested animal tissues into chunks, tend to have high crowns with only a few vertically oriented facets giving them a low OPC.
For example, real organs are not simply chunks of tissue interlaced with blood vessels, but internally structured machines comprising various kinds of tissue all working together to do a job.
The primary infection becomes grossly swollen and painful, killing surrounding tissue and requiring large chunks of affected flesh to be surgically removed.
The procedure removed three inch-long chunks of tissue, one from each lobe of Zadroga's right lung, and James had to spend about a week in the hospital recuperating.
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