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For comparison in initial binding studies, unfractionated tissue homogenates were also prepared by homogenization of tissue in only PBS.
Suspension of tumor cells was prepared from tissue homogenates.
The protein concentration of tissue homogenates was determined using the BSA method as described previously [17].
0.5 mL of tissue homogenates were collected and 1 mL methanol was added.
Malondialdehyde level was also increased significantly, whereas the ratio of reduced glutathione to oxidized glutathione was decreased in tissue homogenates.
Cell lysates or tissue homogenates were centrifuged for 15 min (12,000 g, 4°C).
The reduced GSH content was measured in tissue homogenates and blood [19 22].
These concentrations exceeded those previously found in neuronal tissue homogenates using different techniques.
Ghrelin, estrogen and progesterone concentrations were measured in the ovarian tissue homogenates via ELISA.
There was a very low specific binding in cortical tissue homogenates of control brains.
Figure 5 Strip test of E. tarda EIB202 with turbot tissue homogenates and on-site detection of turbot ascite.
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