Sentence examples for thick consecutive from inspiring English sources

All samples were embedded in tissue-tek (Sakura, Fintek, NL) and snap-frozen in liquid nitrogen before processing to 4μm thick consecutive cryosections.

Thereafter, 4 μm thick consecutive sections were cut from the array block for immunohistochemical staining.

The brain was thawed and cut into 20  μm thick consecutive coronal sections of the white matter using a cryostat (CM 1900; Leica Instruments, Nussloch, Germany).

Using a 1000S Leica vibratome, 50 μm thick consecutive coronal sections, covering the whole striatal region, were obtained and stored free-floating at 4°C in PBS with 0.05% sodium azide.

After incubation for secretome analysis, all segments of RCA and FA were placed in 5% buffered formalin and processed for paraffin embedding: longitudinal 5  μm thick consecutive sections were obtained by a rotary microtome (Microm HM 300, Bio-optica) and stained with Haematoxylin and Eosin (H&E) and Masson's trichrome stain in order to observe and morphometrically assess pathologic changes.

The presence of a discoid meniscus is suggested on MRI when three or more 5-mm-thick consecutive sagittal images demonstrate continuity of the meniscus between the anterior and posterior horns, producing an "extra bow tie" [14].

Five-micrometer-thick consecutive sections were cut and mounted on glass slides.

Briefly, 4  μm-thick consecutive sections were deparaffinised and hydrated through a graded series of alcohols.

Tissue sections were cut into 5- μm-thick consecutive sections and dried at 70°C for 30 min.

For TUNEL assay, tissue samples were embedded in paraffin and cut into 4- μm-thick consecutive sections.

For each tumour, five 4-micron-thick consecutive sections were used for anti-ER, -PR, -ERB-B2, -CK5/6 antibodiesantibodies.