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Sediments and suspended material in the sample was minimized by pumping from an intake tube pulled vertically through about 2 m of the water column at locations about 3.5 to 6 m deep.
Genomic DNA contamination in the sample was minimized with a DNase digestion step.
Genomic DNA contamination in the sample was minimized with a DNase digestion step (Rozen and Skaletsky 2000).
By this way saliva contamination of the sample was minimized and the percentage of squamous cells in the sample was decreased.
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In addition, such devices can reduce ice crystal contamination since manual handling of the sample is minimized.
Because the two-photon excitation is limited to the focal plane, photodamage and bleaching to the sample are minimized.
So, the differences between the samples are minimized.
The principal component equals the direction in which the sum of the distances between the principal component and points (amplitude of the particle motion of every sample) is minimized.
For this reason, heavy plaque sampling was minimized in the present study by taking only a sufficiently visible amount of supragingival plaque.
Blood sampling was minimized to avoid excessive stress and its effect on the metabolic state, as reported previously [31].
The time spent transferring samples is minimized by using a specialized commercial service.
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