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When bruised, the pore surface stains brown.
Heavy dirt or surface stains: wear the item into the shower or spot clean with a lightly damp cloth.
The majority of surface stains were done for 30 min at 4°C in FACS buffer (PBS +0.5% BSA +0.1% NaN3).
Cells were washed of excess surface stains, fixed and permeabilized using Cytofix/Cytoperm™ (BD Biosciences), and stained using anti-IL4 (11B11, BD Biosciences) in permeabilization buffer (FA Buffer+0.1% saponin (Sigma)) at 4°C for 30 mins.
In addition, various permeabilization conditions may individually affect particular surface stains.
Surface staining can be performed before or after intracellular staining but conditions should be tested as new surface stains are incorporated in the assay.
Similar(43)
(B) Surface staining of 293-hSCARB2 cells.
Open image in new window Fig. 12 Surface staining on laboratory specimens after 6 months.
To detect intracellular Bcl6, cells were stained in the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) after surface staining.
A separate set of samples for analysis of GR binding capacity was surface stained as described above but not permeabilized.
Cell surface staining was performed.
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