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One of the drawbacks that impacts negatively on the neuropathological contribution to advancing the field of canine and feline epilepsy is the lack of consensus guidelines for brain sampling, tissue processing, candidate areas, stains and algorithms.
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The areas stained and areas of entire leaves, both in pixels, were used to calculate percentage areas of leaf stained [12] [13].
Using Volocity 3D image analysis software (Improvision, Waltham, MA, USA), we set an intensity threshold to mark only those areas stained with Aβ (Supplementary data, Fig. S1).
Perinecrotic and periulcerative areas stained strongly.
Red areas stained by alizarin red are an indication of calcium deposition.
Moreover, in most patients SRS-positive areas stained at least for the somatostatin receptor subtype sst3.
Areas stained positive for α-SMA were quantified in a parallel fashion.
Most NICC were surrounded by monocytes and neutrophils with areas staining for C3c in surrounding clot.
Digital analysis of the corneal injuries areas stained with fluorescein was used to calculate the size of the wounds.
The analyses consist of measure and comparison of the areas stained by the picrosirius dye and the staining intensity per pixel between the temperatures and normal skin.
The demarcation between areas staining positively and negatively by IHC was sometimes abrupt, as seen for FAP staining in Figure 2A, left.
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