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There, subsequently to each learning trial, 100 non-learning trials were used for estimating mean performance.
When determining multiple interfaces, the described method is applied subsequently to each interface of interest.
PE (Phycoerythrin) conjugated mouse monoclonal antihuman TRAIL or its receptor antibodies were added subsequently to each tube.
To enable the protein to tumble, the equilibration phase was extended (5 ns) after replacing the positional restraints with collective conformational restraints (RMSD), applied first to the whole protein, and subsequently to each of three transmembrane domains separately.
Effluent, EE2, and dilution water were pumped via peristalsis into glass mixing chambers and subsequently to each exposure tank (20 L volume) at a total flow rate of 30 mL/min.
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Video recordings of each trial were watched subsequently to record each visiting bird's species, duration of visit (in seconds), whether or not feeding occurred, and which food was eaten (sunflower seed or peanut).
A fast graph matching approach is used subsequently to extend each ITS to a global atom mapping.
The relative expression values quoted in Figure 2 (now Figure 1 in the revised manuscript) are referenced to a housekeeping gene (Tbp), which encodes the TATA-box binding protein, and then subsequently to P0 for each isoform (ΔΔCT method).
Eventually, the rifting process separated the two viviparid clusters into the four modern groups (i.e. the Congo-, Malawi, Okavango-, and Victoria group; Splits B and C) subsequently to Split A. Each of these groups is (much like in fish) confined to one side of the western branch of the EARS.
Three pairs of individuals were found to be related through the program Relpair version 2.0.1 [ 33] and were determined subsequently to be siblings; in each case, the one with the most complete data was retained.
A customized Perl script (https://dendrome.ucdavis.edu/TGPlone/research-projects/ace-sap/) was used subsequently to identify sequencing primers in each alignment and to mask bases outside the sequence interval defined by these primers whose signal would be due to mispriming.
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