Sentence examples for stock buffer from inspiring English sources

The PMN was carefully removed from the sample and suspended in 10 ml cooled (4°C) phosphate-buffered saline (PBS) stock buffer (diluted 1 10, v/v; 10×PBS stock buffer, without Ca2+/Mg2+, Gibco, Karlsruhe, Germany).

Stock buffer (96.6 μl of 125 mM sodium phosphate, 6.3 mM disodium EDTA, pH 7.5) was then added to each tube, vortexed and re-centrifuged as above.

The mobile phase was prepared with the stock buffer mixed with methanol in the ratio of 8 2 (v/v) and degassed after mixing.

The stock buffer for the mobile phase was comprised of the following: 0.1 M sodium acetate, 0.1 M citric acid and 27 μM disodium ethylene-diamine-tetra-acetate (EDTA) dissolved in 1 L of deionised water.

Prepare 0.5 μg/μL trypsin or a different protease in H2O or a stock buffer specified by the vendor, dispense into aliquots of 10 µl or less, and store at −80 °C.

The CMCT stock buffer was 42 mg/mL CMCT in BMK buffer.

All stock buffers were stored at 4°C and were kept for 1 month.

Stock buffers were sterilized by filtration using a VWR International syringe driven 0.2 micron cellulose acetate membrane filter.

Stock buffers were prepared at 1 M in deionized water and pH was adjusted by addition of appropriate molar ratios of conjugate acid and conjugate base, or by empirical adjustment with HCl or NaOH.

Hybridization and washing solutions were prepared from two stock buffers: 20X SSC (3M NaCl, 300 mM sodium citrate, adjust with Citric Acid to pH 7.0) and 1X Maleic Acid (0.1M Maleic Acid, 0.15M NaCl adjusted with NaOH to pH 7.5).

Two stock buffers were prepared from which a range of GdnHCl (guanidine hydrochloride) concentrations could be produced: fluorescence buffer A – 20 mM Hepes and 50 mM NaCl, pH 7.4; fluorescence buffer B – 20 mM Hepes, 50 mM NaCl and 6 M GdnHCl, pH 7.4.