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The researchers took steps to rule out this possibility, but Dr. Goldstein said those steps might not have been adequate.
In a statement responding to the new papers in Retrovirology, Judy A. Mikovits, director of research at Whittemore Peterson and the senior author of the Science study, said her team took extensive steps to rule out P.C.R. contamination and also focused on other approaches to finding XMRV.
However, we took steps to rule out any obvious associations with memory.
It is possible that E- and Iplasma do have blue-copper proteins in their genomes because gaps remain in their assemblies, but we took steps to rule out this possibility (see Methods section).
Approximately 20%% of the cultures required additional process steps to rule out potential pathogens; these additional steps resulted in additional labor (0 35:00 – 0:40:00) and supplies.
But the authors did not take any steps to rule out the possibility that the difference between these groups may be neurovascular in nature, and nothing to do with neuronal activity.
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An observational study would require "enrolling sufficient numbers of unvaccinated children and matching them with vaccinated children of the same age, gender, ethnicity, and geographic location — a necessary step to rule out chance findings" and would be prohibitively difficult and time-consuming, if not impossible.
Negative control reactions were performed without reverse transcriptase during a cDNA synthesis step to rule out any genomic contamination.
PASS (Campagna et al., 2009) implements another solution: a filter is introduced before the full extend step to rule out areas that have too many differences with the query sequence.
Read the next few steps to find the rules.
That is, he is descended from Britain's the House of Hanover – the German protestant dynasts who stepped in to rule Britain in 1714.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com