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One of the metabolic steps consists of the decarboxylation of the α-keto acids of leucine, isoleucine, and valine, respectively.
The explicit time for discrete steps consists of adding a real parameter to each node state.
The implicit time for discrete steps consists of adding a probability to each transition of the transition graph in the case of non-deterministic transitions (asynchronous case).
Subsequent steps consists of using Megablast and a junction database to identify overlapping, spanning, and split reads to detect fusions that are further filtered using SnowShoes-FTD author provided false positive list.
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The three steps consist of aggregation, optimization, and control.
The second and the third steps consisted of cloning the EYFPBax and ECFPBax cDNA.
The final steps consisted of 20 25 cycles of 94° (5 sec).
All washing steps consisted of 1 minute of flow, followed by 5 minutes of incubation.
The following steps consisted of masking the repeats, vector and organelle sequences.
The sample QC steps consist of five scripts [ Fig. 1 (in light blue, see online version)].
ChIP-seq will generally have two intermediate steps consisting of read mapping and peak calling.
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