Indeed, fewer protease inhibitors genes, six and two, were induced by both isolates at the early and later stages of inoculation, respectively.
Our results show that there are 21 probe sets (representing up to 19 genes) are commonly up-regulated and a number of genes that are specific to early, late or very late stages of inoculation.
We found that efficiency of BSMV-induced gene silencing varied greatly with the locations and the stages of inoculation, and inoculation with BSMV on wheat spikes from heading to flowering stage by spike-rubbing five times showed the most effective silencing of PDS in wheat spikes (Table 1).
This profile was indicative of an enhanced reaction of compatibility between the host and the pathogenic isolate, compared to the non-pathogenic one, at the later stages of inoculation and it suggests that the plant may already react to the invading pathogens in the first 96 h.
The insects were transferred to rice seedlings grown to the two- to three-leaf stage for inoculation of RDV-D84.
However, by later stages of post inoculation, the inoculated roots of the partially susceptible cultivars failed to increase abundance of any transcripts of known defense-related genes.
The tendency was more prominent for constitutive samples and at early stages of the inoculation; samples taken from inoculated roots at 15 and 28 dpi were not separated on a susceptibility basis.
