The concentration was adjusted to 0.1 mL L−1, corresponding to approximately 10−9 M. The metabolites were added to the inoculant containing Azospirillum at the time of inoculation, either when applied to the seeds, or by spraying.
Non-inoculated plants served as control at the time of inoculation.
Survival of Salmonellae on and in tomato plants from the time of inoculation at flowering and early stages of fruit development through fruit ripening.
Four pregnant females ranging from gestational day (GD) 41 to GD90 at the time of inoculation were selected because the gestation of rhesus macaques is ~165 ± 10 days, and IA inoculation is feasible starting at GD40.
Under the optimum growth conditions determined for the single culture fermentations, i.e. 378.4 mM NaCl, 34.1 mM Ca-acetate and 39.9 mM Ca-lactate, mixed culture fermentation was undertaken by varying the time of inoculation of the yeast strain.
In contrast, fetal loss or infant CMV-associated sequelae occurred in four rhCMV-seronegative pregnant macaques that were CD4 T-cell depleted at the time of inoculation.
To minimize environmental variation at the time of inoculation, the 'cut-panicle inoculation' method was created (Miyagawa and Kimura 1989).
Other references show that food preferences for freshwater detritivores are related to the time of inoculation with microorganisms [[29],[30]].
At the time of inoculation, the number of rhizobia in the inoculum was estimated using plate count method.
The medium and the substrate were mixed at the time of inoculation at 108 spore/ml and incubated at 30°C under static condition.
The Natto peptide was added to the cell cultures at the time of inoculation or 4 h after the inoculation (approximately 1 × 107 cells/mL).
