Sentence examples for spindle damage from inspiring English sources

These results revealed that down-regulation of Bub3 could override the metaphase arrest induced by nocodazole, indicating that Bub3 is essential for meiotic arrest of mouse oocytes in response to spindle damage.

Previously, it has been shown that cells depleted of Mad2, Bub3, Rae1, BubR1 or CENP-E are unable to sustain a pro-metaphase arrest in the presence of spindle damage [23], [24].

To further explore the role of Bub3 as a spindle checkpoint protein, we confirmed that the optimal dosage of nocodazole that was able to block the MI-AI transition but not GVBD in meiosis without apparent spindle damage was 0.04 µg/ml [32], [33] (Supplementary material Fig. S2 ruled out any side effects of nocodazole on meiosis I).

Treatment with higher doses of nocodazole leads to increased missegregation events upon release also in control oocytes in this strain background (data not shown), therefore 200 nM nocodazole were used to assess the capacity of mad2+/− oocytes to respond correctly to spindle damage.

Yuan et al. [ 31] showed that MAD2L1 and BUBlB, known as spindle damage checkpoint genes, were overexpressed in breast cancer tissues.

To assess the checkpoint response to spindle damage, we compared MPM2 staining after nocodazole treatment in cells expressing three different BubR1 constructs.

Division arrest may be initiated by severe DNA damage, spindle perturbation or even heat shock and should not be revoked until the problem is solved.

We asked whether our model has the capacity to regenerate a damaged spindle.

The prediction of stable cutting regions represents an important issue for the machining process, which may otherwise give rise to spindle, cutter and part damage.

Overall, our data show that checkpoint proteins are only recruited at kinetochore in case of damage in spindle assembly.

This is consistent with tetraploidy in Slbp mutants being caused by prolonged arrest at the spindle checkpoint due to damage to the genome.