siRNA 2 showed the highest (98%) silencing effect on protein level and siRNA 4 with 83.1% at the mRNA level.
Silencing effect of TsNd transcription and expression was determined by real-time PCR and Western blotting, respectively.
Also, two pieces of small interfering RNA (siRNA) were designed and synthesized by in vitro transcription, which exhibit specific and efficient silencing effect on mce1a expression.
The silencing effect became significant 66 h after siRNA transfection (data not shown).
The effect of DNA methylation on gene responsiveness could be explained by a simple transcriptional gene silencing effect [ 22, 23].
This is confirmed by the finding that the use of PLA/siRNA at the higher weight ratio than 5 : 1 (e.g., 10 : 1) did not further increase the gene silencing effect, but adversely lowered this silencing effect.
Additionally, the silencing effect lasted for at least 72 h.
Moreover, the results generated had comparable off-target silencing effect.
This has a real silencing effect," she told the BBC.
In-vitro evaluation of silencing effect was performed by using RSV infected Vero cell line.
