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To assess the significance of sampling by this approach, multiple probe microdialysis sampling was used to monitor drug absorption in the stomach.
Non-parametric bootstrap trees were analyzed to assess the significance of sampling variability.
We then calculate the significance of sampling the sum of the scores of the genes from the larger pseudo pathway.
Despite these empirical predictions, the nature and significance of sampling effects for single cell expression profiling have not been systematically studied to date.
The purpose of this work was to systematically evaluate the presence and significance of sampling effects in PCR-based global amplification-based single cell expression profiling.
One promising technique for mRNA amplification from individual cells, which combines global exponential and linear amplification, has been shown to produce very low levels of noise and highly reproducible data and may limit the significance of sampling effects when profiling rare transcripts [ 22].
Similar(54)
For (A – C ) ANOVA analysis was used to determine significance of samples with Bonferroni's post-test to determine significance between groups.
In case of Salmonella contaminated fresh produce as well as pathogenic bacteria on fish, studies indicate the significance of sample preparation [ 17– 17].
Each reaction mixture on each optimization plate for each target was run in triplicate or quadruplicate in order to bolster the statistical significance of sample assessments.
Furthermore, the significance of sample composition (cancer and normal tissue) on subtype classification was evaluated and the persistency of the subtype classification in samples with different Gleason score from the same patients was assessed.
For clinical samples, the statistical significance of sample Ct value differences were assessed using an ordinary one-way ANOVA test with Dunnett's multiple comparisons test using Prism 6.0.
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