Sentence examples for several washes in from inspiring English sources

After several washes in TBST buffer and incubation for 1 h at RT, antibody binding was detected by using horseradish peroxidase (HRP -conjugated secondary antibody, at RT.

After several washes in PBS, the labeled cells were scanned by LCSM (510 Meta Duo Scan, Carl Zeiss, Oberkochen, Germany) using 545-nm (He-Ne) excitation.

After several washes in TBS-Tween, membranes were incubated with goat anti-rabbit HRP-conjugated secondary antibody (1 2000, ZSGB-BIO, China).

After stopping the reaction by several washes in PBS.

After several washes in PBS, sections were incubated with Alexa488-conjugated secondary antibody (1∶50) [32].

After 24 h, testes were washed in 70% ethanol, followed by several washes in 100% ethanol.

After several washes in PBS, sections were mounted on slides with Vectashield (Vector Laboratory).

Free aldehydes were blocked with several washes in PBS supplemented with 50 mM glycine.