Sentence examples for multiple washes in from inspiring English sources

Slides were then washed twice with 70% formamide, 10 mM Tris HCl pH 7.0 7.5, 0.1% BSA followed by multiple washes in PBS.

Excess paraffin was removed by multiple washes in xlyene.

Following multiple washes in PBS, biotinylated secondary antibodies (1∶200 dilution, H+L, Zymed Laboratories, US) were incubated at room temperature for 30 minutes.

After multiple washes in TBST, the membrane was incubated with the corresponding secondary antibody conjugated with alkaline phosphatase (Sigma-Aldrich) diluted in 2.5% NFM-TBST, washed, and developed with BCIP/NBT (5-Bromo-4-Chloro3'-Indolylphosphate p-Toluidine salt/Nitro-blue Tetrazolium Chloride) according to the manufacturer's instructions (Pierce, Rockford, IL).

Following multiple washes in TBST, bands were visualized using Visualizer Western Blotting Detection Kit (Millipore, Billerica, MA) according to the manufacturer's protocol.

Following multiple washes in PBS plus 1% Tween 20, anti-Etag-HRP antibody (Bethyl) was added at a 1 10,000 dilution in PBS plus 5% non-fat dry milk for 1 hr at RT.

Finally, all the CNTs-supported NPs were extracted from [BMIm][BF4] using high-speed centrifugation and decantation, followed by multiple washing in acetone aided by bath ultrasonication.

After multiple washes, blots were incubated in goat anti-rabbit HRP (1∶50,000; Abcam Inc., Cambridge USA) in 5% BSA for 90 minutes at room temperature.

Following multiple washes, samples were mounted in Vectashield mounting medium (Vector laboratories, Burlingame, CA).

The lower part is a case in which multiple washing and transporter robots are employed.

After multiple washing steps, the erythrocytes were resuspended in 50 µl PBS/fetal calf serum containing 1∶100 fluorescein (FITC -conjugated aFITC -conjugatedb′)2 fragment donkey affinity-pureG (H+L), (Jackson ImmunoResearch Laboratories) and 1∶100 propidium iodide fragmentin.