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Exact(7)
Genes with available expression estimates from all methods and which were mapped to sequencing estimates (requiring 1+ read) were included to compare exon array expression estimates to ultra high-throughput sequencing estimates.
To compare the concordance between GeneBASE or GeneBASE-xhyb and sequencing estimates, we transform exon array expression estimates so that they have the same distribution as sequencing estimates (quantile transformation).
We present the results for mouse exon arrays and compare them to ultra high-throughput sequencing estimates of liver, muscle and brain tissues.
Using quantile normalization, we transform microarray estimates, taking the median across sample replicates, to have the same distribution as the sequencing estimates.
If the GeneBASE-xhyb estimates are no more concordant with sequencing estimates than the GeneBASE estimates, then we would expect T to be centered at 0. If GeneBASE-xhyb tends to show smaller deviations from the sequencing estimates than GeneBASE, then we expect T to be shifted to the right.
On the other hand, if GeneBASE-xhyb is more concordant with sequencing estimates, we would expect positive values of T. Therefore, we carry out a Wilcoxon signed rank non-parametric test of the null hypothesis T=0 against the alternative, T > 0. From the distribution of T values, we see enrichment of T values above zero.
Similar(53)
For DNA sample NA18507, next-generation sequencing estimated the CCL3L1 copy number to be 5.7(25) whereas our ddPCR system estimated 6.05.
Recent studies of oral microbiota using high-throughput sequencing estimated the number of species-level phylotypes between 540 and about 10,000 [ 9- 11].
Only about 100,000 people have paid to have their genes sequenced, estimates Eric Topol of the Scripps Research Institute in San Diego.
The gateway, upon reception of all the sequences, estimates the values in the channel matrix.
Although the minimization of (15) will maximize (4), the resulting sequence estimates will only be optimal in the sequence sense.
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