Sentence examples for sequencing directions from inspiring English sources

Below is the next-generation sequencing read alignment, small and capital letters correspond to different sequencing directions.

We first cleaned raw sequence reads by removing exact duplicates from both sequencing directions.

We first cleaned the raw sequence reads by removing the exact duplicates from both sequencing directions.

SSR frequencies were also calculated for each cDNA library to identify frequency differences between tissue/stage types and between sequencing directions (i.e. between 5′ and 3′ ESTs).

We first cleaned the raw sequence reads by removing the exact duplicates from both sequencing directions with the FASTX Toolkit [ 61].

For ESTs from other tissues and sequencing directions, the libraries listed in Table 1 were used (CLFL for leaves, CMFL for male buds and CFFL for female buds).

Skewed sequencing direction was highly associated with errors.

The sequences in each library were divided into separate groups on the basis of their sequencing direction (3′ or 5′ ESTs) where sequencing direction data were available.

Tissues, dbEST library identifier, sequencing direction, and library descriptions are provided.

Stacked reads i.e. redundant reads sharing the same start and end coordinate, sequencing direction, and sequence were merged into one.

Thus in the case of non or partially overlapping forward and reverse reads, the resolution of the approach is limited by the less effective sequencing direction [ 46, 47].