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A list of the sequencing trend for each cDNA library is presented in Table 3.
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For those time series of small time span but large sequence trend changes, time segment calculated by PAA_ERR would get very close to the original sequence, and resulted in poor final segment results.
However, known natural sequences usually represent only a subset of all existing binders biasing the observed sequence trend.
In most cases, they have alternate R and Y-dominant stretches along their genome sequences (Trend III, Table 2).
Such analyses have been thought to be an effective way to identify important sequence trends and probable poly(A) signals [ 7, 12, 27].
In case the observed sequence trends are caused by the selection for cleavage promoting and stabilizing amino acids in the beginning of HEG coded proteins, then it is interesting to note that alanine has been chosen from the set of six amino acids with similar properties.
There is however a growing trend towards sequencing large number of samples [4], and given the anticipation that the cost for NGS will drop further, it will soon be possible to do so.
This technical advancement has initiated the trend of sequencing multiple samples in different conditions or environments to explore the similarities and dissimilarities of the microbial communities.
Metrics are considered consistent if they consistently ranked perfectly assemblies as better; fully consistent metrics are consistent metrics with similar monotonic trends by sequencing depth and read length for perfect assemblies.
Table 4 shows the concordance as a function of number of "anchor" markers when 50% of the samples where sequenced; the same trend was observed when a smaller proportion of samples were sequenced and used as the reference (data not shown).
Though the control regions of four mt-genomes were not completely sequenced, this biased trend is obvious even without the control region sequence.
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