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Nucleotide sequencing of the purified PCR products was performed using the ABI PRISM BigDye Terminator cycle sequence reaction kit and the automatic DNA sequencer ABI PRISM, model 377 (Applied Biosystems, Foster City, CA, USA), at the Unidad de Genómica, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid (UCM), Madrid, Spain.
DNA products were then purified by ethanol precipitation and sequence reaction products analyzed on the ABI Prism 3100 automatic sequencer (Applied Biosystems).
Sequence reaction mixtures were analyzed on an ABI 3730 automated DNA sequencer.
Sequence reaction products were analysed on the ABI 3700 automated DNA sequencer (Applied Biosystems, Carslbad, CA, USA).
After purifying of the product of sequence reaction, the sequences were determined using ABI 377 or ABI 3700 sequencer according to the protocol by manufacturer.
The same trend follows from the consideration of a two-step sequence reaction mechanism.
Similar(28)
Sequence reactions were analyzed with an Applied Biosystems 373XL sequencer.
Sequence reactions were analyzed on an Applied Biosystems 3130.
Sequence reactions were performed as reported in [ 17].
Contamination or mix-up of extractions, PCR samples or sequence reactions: The sequences are verified by multiple accessions of the species, and multiple PCR and sequence reactions.
After that, sequencing reaction was performed using ABI 3500XL Genetic Analyzer sequencing machine.
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