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PCR products were excised from agarose gel, purified with QIAquick gel extraction kit (Qiagen, Valencia, CA, USA), and sequenced reaction with anti-sense primer and DYEnamic ET Dye Terminator (Amersham Biosciences, Piscataway, NJ, USA), according to the manufacturer's instructions, in 4 replicates.
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After the bespoke miniature lab had set hard, the printer could then inject the system reactants, or "chemical inks", to create sequenced reactions.
After that, sequencing reaction was performed using ABI 3500XL Genetic Analyzer sequencing machine.
Sequencing reaction products were purified with the Sequencing Reaction Cleanup Kit (Millipore, Billerica, MA) and resolved by electrophoresis on a 3730 DNA Analyzer (Applied Biosystems).
The PCR products were purified for sequencing reaction by using a sequencing primer (5'-ATGCTCGGGGATCCGAATTC-3').
Sequencing reaction products were purified prior to electrophoresis using the Montage SEQ96 Sequencing Reaction Kit (Millipore Corporation).
Sequencing reaction was performed using ABI Prism Big Dye terminator kit (Perkin-Elmer).
Sequencing reaction was done using BigDye Version 2.1 Applied Biosystems, Foster City, CA).
Sequencing reaction was carried out using Applied Biosystems BygDye™ protocol.
Sequencing reaction was performed with a BigDye Terminator v3.1 Ready Reaction Cycle Sequencing kit (Applied Biosystems).
Sequencing reactions were run on ABI3730 sequencers at College of Agricultural Sciences, Genomics Facility (CGF) of UC-Davis.
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