Sentence examples for seconds with cold from inspiring English sources

Pre-flushing of 30 seconds with cold water was the most effective method to prevent bacterial accumulation in the tested non-touch fittings.

Flushing for 10 seconds with cold water after use or 30 seconds flush before use were both the most effective measures to prevent non-touch fittings from biofilm formation over a period of 20 weeks.

Rinse the hair for a few seconds with cold or cool water (depending on your clients sensitivity).

Especially in teen years, pimples can be a problem so rinse your face with warm or even hot water for one minute and then rinse it for thirty seconds with cold.

For postnatal ages, animals were transcardially perfused first with cold PBS and then with cold 4% PFA before collecting the brains.

For staining of cryo-sections, sections were fixed first with cold acetone (10 min), before probing with anti-Irga6 antiserum 165/3 or anti-TCR Vβ8.1/8.2 mAb (BD Pharmingen, CA, USA).

For the in situ GFP-LC3 fluorescence analysis, mice were euthanized by carbon dioxide asphyxiation, then immediately perfused through the left ventricle first with cold phosphate-buffered saline (PBS), followed by 4% paraformaldehyde in PBS to fix the tissue.

The reaction was terminated on ice by washing first with cold 0.1 M citric acid/0.2 M sodium phosphate buffer alone, followed by five washes in 0.15 M phosphate-buffered saline, pH 7.4.