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An extended dynamic range scan was then accomplished by performing a primary scan at 100% laser power and a secondary scan at 10% power; the former used to calculate intensities for non-saturating features, and the latter used to calculate intensities for saturating features.

The primary scan forms the basis of the analysis, whereas the secondary scan is used to correct the intensities of spots with saturation in the primary scan.

This secondary scan procedure was equivalent to installing the a priori QTL as covariates, thereby attributing a large portion of the overall variation to these QTL and allowing for detection of additional loci controlling the remaining variation.

For RDP4, we use the RDP (Martin and Rybicki 2000), Geneconv (Padidam et al. 1999), BootScan (Martin et al. 2005), MaxChi (Smith 1992), Chimaera (Posada and Crandall 2001), SiSscan (Gibbs et al. 2000), and 3Seq (Boni et al. 2007) methods with the default settings during both the primary scan (identification of recombination) and secondary scan (verification) phases.

When we considered only strains homozygous for the N2 allele at the marker closest to ppw-1 (4,175,488), or when we included this marker as a covariate and repeated the linkage mapping (see Figure 1B), no significant QTL were recovered from the secondary scan.

We therefore propose a procedure where two scans are obtained for each of the red and green channel; a primary scan with the lowest intensities within the usable range of the scanner and a secondary scan with the intensities of the brightest spots just below the level of saturation.

Similar(54)

Also by secondary scanning electron microscopy (SEM) and back scattered electron (BSE) the morphology of samples and their particle size and shape are studied.

Main QTL and corresponding mean trait values from the primary scans were used to obtain estimates of residual empirical thresholds to search for additional QTL via secondary scans [ 46].

Consequently, the remaining unassigned fraction of CT variation was analyzed using secondary scans of the residual variation after statistically removing the effect from the chromosome 12 locus; subsequent genome-wide scans identified markers C14M96 and C14M68 on chromosome 14 (LOD scores of 2.388 and 2.392 respectively).

A temperature melt performed from 20 to 80°C was followed by the acquisition of a secondary structure scan.

In the context of cognitive radio [7, 8], where a secondary user scans the spectrum assigned to primary users for a transmission opportunity (spectral sensing), the high spectral definition of FBMC is especially valuable.

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