The latency to fall off the rotating rod per rotation speed level was recorded as a measure of motor function.
After the training session, mice were placed on the rotating rod with a linear increase in rotation speed from 2.5 to 48 rpm over a 5-min period.
Mice were placed on the middle of the rotating rod, its body axis being perpendicular to the rotation axis, and its head against the direction of rotation.
Animals were placed on rotating rod at 5 rpm with graded increases in rotational velocity over 5 min to a maximum of 49 rpm.
Instead of putting them in driver's seat, the researchers placed the animals on a rotating rod to check their balance.
It utilizes a disk of rotating rod made of glassy carbon as a working hydrodynamic electrode.
After seven days of stimulation, mice were able to walk further down a rotating rod than mice which had not had brain stimulation.
Briefly, mice were place on a rotating rod with a initial speed of 4 rpm.
During the training period, mice were placed on the rotating rod starting at 5 rpm and gradually accelerated to 50 rpm at a rate 0.15 rpm/s.
Each trial lasted a maximum of 5 min, while the rotating rod underwent a linear acceleration from 4 to 40 rpm.
The speed of rotation was gradually increased (0,3 rpm/sec) and the rodent's ability to remain on the rotating rod (time to the first fall) was recorded.
