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RC24/EDC mixture was added to the magnetic beads, mixed by 10 sec vortexing and incubated on a rotating mixer at ambient temperature overnight.
Coarse aggregate was subsequently added to the rotating mixer and mixing was continued for further 2 min.
Dry mixing of the aggregates and cement was done for 2 min and then water was added gradually in the rotating mixer and allowed to mix for 5 min.
To produce a transient magnetic pull, without the need for a micron size electro-magnet, we rotated a rare earth magnet on a rotating mixer beneath the culture while the culture was suspended above the magnet (Figure 1A).
Cell lysates from neutrophils or basophilic cell line (KU812, ATCC® # CRL-2099™) used as positive control were sequentially incubated with 2 µg/ml of human IgE (Diateck, Oslo, Norway) for 16 h at 4°C in a rotating mixer, followed by protein G sepharose-coated beads (Amersham-Pharmacia) conjugated with mouse mAb anti-human IgE (Pharmingen) for 2h at 4°C.
Cell lysates from B/TSM or basophilic cell line KU812 (positive control) were pre-cleared with protein G sepharose-coated beads (Amersham-Pharmacia) for 2 h at 4°C in a rotating mixer, followed by incubation with protein G sepharose-coated beads conjugated with 2 µg/ml of murine anti-human Fc��RIα mAb 15/1 or isotype mouse IgG1 monoclonal antibody (MOPC 21) for 16 h at 4°C.
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We also explore the role of particle shape in mixing performance and use DEM to test whether Froude number scaling is suitable for predicting scale performance of rotating mixers.
Rotating pan mixer is similar to mixer which has common concrete plants.
In experiment UHPC was prepared with high intensity mixer "EIRICH R02", then mixture was modified and prepared in rotating pan mixer "Zyklos ZZ50HE".
Thereafter, the mixture underwent hydrothermal treatment at 130 °C for 6 h and was stirred at 600 rpm using a Teflon-lined autoclave equipped with adjustable rotating speed mixer.
I, of course, never "caught" the drum of the cement mixer rotating.
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