The eluate was monitored with a Shodex RI-101 (Kawasaki, Japan) refractive index (RI) detector.
The column effluent was monitored by a Refractive Index (RI) detector.
Peaks were detected by using a RI detector (Waters 2414, Waters Corporation, Milford, MA, USA).
GPC was equipped with a Waters 6000 solvent delivery system, RI detector, and a Rheodyne injector.
Actually, the RI detector for 2 detected polymers at about 0.5 min after sensing UV absorbance.
Fig. 2 SEC traces of 1 (a) and 2 (b) with RI detector.
Molecular weights of 1 and 2 shown in Table 1 were determined by SEC with RI detector (Fig. 2a, b).
Molecular weight of PS was found to be 310 kDa using HPLC-SEC coupled to refractive index (RI) detector.
Total mannan and glucan were determined by HPLC using an isocratic method and a Refractive Index (RI) Detector.
Experimental measurements were conducted using a high performance liquid chromatography (HPLC) equipped with size exclusion chromatography (SEC) columns and a refractive index (RI) detector.
A Shodex RI-101 RI detector and an Ultimate 3000 VWD 3100 variable wavelength ultraviolet detector coupled to the HPLC unit were used to quantify the metabolites.
