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The pyroelectric (PE) detector, a polyvinylidene diflouride (PVDF: 52 micron, MSI DT1-028 K/L), is very sensitive to small changes in the heat flux; this PE detector was fixed with silicon glue to a Perspex substrate.
Since propidium iodide was used to stain the nuclei, fluorescence was measured using the R-phycoerythrin (PE) detector, which uses the 576/26 nm bandpass filter.
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In order to quantify gene expression for each RNA sample, multiplex real-time PCR was run on 96-well plates using ABI 7700 Sequence Detector (PE Biosystems) according to methods previously described [42].
The PCR amplification cycle was 50°C for 2 min and 95°C for 10 min, followed by 40 cycles of 95°C for 15 seconds and 62°C for 1 min. Levels of FAM and TET fluorescence were determined and allelic discrimination was carried out using the ABI 7200 Sequence Detector (PE Biosystems).
The Y value for each cDNA sample and target sequence was divided by the Y value from the housekeeping gene (18S) for that particular sample to derive a Δ Ct value (PE-ABI; Sequence Detector User Bulletin 2).
Finally, they concluded that the incremental rate of subsegmental PE detected by multiple detector CTPA might not be clinically important [ 44].
Relative μ-calpain expression levels were measured by ΔΔCT method (PE-Applied Biosystems; Sequence Detector User Bulletin).
In cases where PE is suspected, multi-detector CT angiography sensitivity ranged between 83 and 100%%, and its specificity ranged between 89 and 97 % [ 29], although this study did not provide specific data for the ICU population.
When the frequency was increased, the sample became thermally thick, and the thermal waves penetrated into the sample; the detector then produced a PE signal, and this PE signal decreased exponentially with increasing frequency modulation.
Data were analyzed and processed using Sequence Detector version 2.3 (PE Biosystems).
Samples were analysed in triplicate using Sequence Detector version 2.3 (PE Biosystems), using the comparative threshold method.
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