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The genetic structure was inferred with Amplified Fragment Length Polymorphisms (AFLPs; [ 56]), a high resolution marker capable of resolving intraspecific differences such as those expected among different ecotypes.
With such high resolution marker coverage, the authors successfully identified a highly significant association for the SNPs in the candidate genes.
All analyses were contrasted with the genetic structure as inferred by Amplified Fragment Length Polymorphisms (AFLPs; [ 56]), a selectively-neutral, high resolution marker system that can generate a high number of markers distributed genome-wide [ 57, 58].
We focused on mortality as death is very reliably recorded, and in the UK, routine mortality data include the postcode of residence which provides a high resolution marker of location for classifying whether an individual was flooded or lived close to a flood area.
Similar(56)
Despite this generalization, the subsequent usage of "high resolution" markers (microsatellites and inter simple sequence repeats [ISSR's]) failed to resolve any further differentiation.
Further it is possible that higher resolution markers would allow us to differentiate A. paupercula and A. purpurea as is discussed below for A. acuta.
Higher resolution markers such as microsatellites may be useful in indicating the pathways by which non-native snails are spreading rapidly through parts of the U.S. We sampled native Pomacea paludosa in Florida and non-native apple snails in Florida, Georgia, Texas, California, Arizona, Hawaii, Cuba, and Guadeloupe in the Lesser Antilles [see Additional file 1].
High throughput sequencing is particularly suited for high-resolution marker discovery and linkage mapping [ 6, 7].
In this study, we were able to genotype colonies of Acropora millepora from the reef flat surrounding Heron Island (GBR) by a high-resolution marker, microsatellites, and an additional molecular marker, intron 4 500 of a carbonic anhydrase isoform.
The full list of SNPs and their variants in all the genomes is provided in Additional file 3. We created a tree from the alignment of high-resolution marker SNPs identified in the 210 V.
Next generation sequencing technologies coupled with high-resolution marker genes such as species-specific DNA barcodes--environmental barcoding--can address data rarity and low taxonomic resolution often crippling biodiversity analysis.
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