Field experiments on GM cottons in eastern Australia were commonplace, but even there, regulators required confirmation that the containment practices already in place (buffer zones and isolation distances) were effective.
However, this approach requires confirmation that the R4 plasmid inserted locus is not subject to genomic positional effects.
Because the analysis required confirmation that the same strain was present in multiple infants, we deployed a genome-resolved sequencing-based approach.
These are: Recruitment: requires confirmation that the study participant meets the inclusion criteria and summarises the patient's admission details including the last 7 days.
The situation of overlapped substrate bands required confirmation that relaxation of the most highly supercoiled substrate topoisomers did not generate relaxed topoisomers that overlapped with other substrate topisomers (e.g., s 14 did not relax to form s 9 to s 13).
Surprisingly, PS1 holoprotein in the nonrescued Pen-2 KO line could also be biotinylated (data not shown), thus requiring confirmation that the biotinylation reagent had not passed the cell membrane by probing for the cytosolic protein GAPDH.
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