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No significant differences were observed between HAD-1 and HAD-2 or between LAD-1 and LAD-2 rats, indicating that the replicate lines performed similarly in this study.
Yet previous behavior experiment and the consistent GFP expression patterns among replicate lines strongly suggest that the observed signals are real.
Moreover due to genetic drift, allelic variation present within the base population might differ between replicate lines in selection experiments or between inbred lines often used in Drosophila association studies.
Random effects were family nested within line, replicate lines nested within linetype, and the sex-by-line linetype) interaction term (the number of familiesex-by-line linetypeht linesex-by-line linetype, 28, 33, 28 & 28 for linteractionugh 8 respectermly; nothelinumbersignated 1, 2, 4 & 5 were coftrol lines, while 3, 6, 7 & 8 were selected lines).
For each experimental condition, we used six replicate lines.
Replicate lines were randomly assigned to two treatments.
We chose the conditions of the experiment (number of generations, replicate lines, etc).
The ancestor was evolved in 114 replicate lines at 42.2°C for 2000 generations.
The analysis addressed age-independent differences among the selective regimes using multiple replicate lines per treatment.
For each replicate, lines connect means, and shaded areas show interquartile ranges.
Replicate lines with low genetic diversity were generated through a series of population bottlenecks.
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